ELISpot: Principles of the technique (1)

Slide: 1, 2, 3, 4, 5, 6

First step in ELISpot

The bottom of each well of the plate is coated with catching (primary) antibodies that are specific to the protein of interest in our assay (eg. a cytokine, antibody, surface marker, receptor, etc.). Next, the cell cultures are set in ELISpot plates. Stimulated cells secrete proteins of interest, which are bound to the well bottom by the catching atibodies. The binding is specific, therefore only molecules of interest are bound to the bottom membrane. More...

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