ELISpot: Principles of the technique (5)

Slide: 1, 2, 3, 4, 5, 6

Fifth (and last) step of ELISpot

In the place of the immunenzymatic reaction, a colour spot emerges. It is assumed that one spot denotes one cell secreting protein of interest. In the case of rapidly dividing cells, or when the cells are cultured over a longer period of time, the term "spot forming unit - SFU" seems more appropriate. On the left-hand side, ELISpot results from a culture with majority of cells secreting protein of interest (in this case interferon-γ). On the right-hand side, a culture, where significantly less cells produce IFN-γ. More...

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