ELISpot: Principles of the technique (3)

Slide: 1, 2, 3, 4, 5, 6

Third step in ELISpot

The next step is to add enzyme (e.g. alkaline phosphatase - ALP, or horseradish peroxidase - HRP) that is coupled with streptavidin. Streptavidin binds covalently with biotin - the marker of the secondary (detection) antibody. Multiple washing of the plate removes the excess of enzyme that remains unbound. More...

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