ELISpot: Principles of the technique (2)

Slide: 1, 2, 3, 4, 5, 6

Second step in ELISpot

After finishing the cell culture, the content of the wells is removed and the plate is washed thoroughly. At the well bottom, only proteins remain that are bound to the primary antibody. Now, we add biotinylated secondary antibory, which binds to another epitope of the same protein. More...

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